rabbit anti cb1r antibody  (Cayman Chemical)

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: The list of primers sequences

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques:

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: CIH promotes overexpression of CB1 receptor in colon tissue. a-b The expression of CB1 receptor in colon tissues was detected by immunofluorescence. c RT-PCR was used to detect the mRNA expression level of CB1 receptor in colon tissues. d-e The expression level of CB1 receptor protein in colon tissues was detected by western blot. * P < 0.05, ** P < 0.01,*** P < 0.001, compared with the Control group; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with the CIH group

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Over Expression, Expressing, Immunofluorescence, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: Inhibiting CB1 receptor mitigated the pathological damage of colon induced by CIH. Representative image of HE staining (100 µm) and transmission electron microscopy (20 µm) of colon tissue. TEM, transmission electron microscopy

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Staining, Transmission Assay, Electron Microscopy

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: Effect of inhibiting CB1 receptor on intestinal microbiome of OSA mice. a OTU Wayne chart; b - c Chao1 and Observed_species indicate the richness of the community; d - e Shannon and Simpson indicate the diversity of the community; and f Pielou_e indicate the evenness of the community. g PCoA principal component analysis based on weighted_unfirc distance. h 3D. * P < 0.05, ** P < 0.01

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques:

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: Inhibition of CB1 receptor alters the gut microbiome composition induced by CIH. a Intestinal microbial composition of top 10 phylum level. b The intestinal microbial composition of the top 10 families. c Intestinal microbial composition of top 10 species level. Difference analysis of flora at d – f phylum level, g–j family level, and k – m species level. * P < 0.05, ** P < 0.01, compared to the Control group; # P < 0.05, ## P < 0.01, compared to the CIH group

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Inhibition, Control

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: CB1 receptor inhibition may ameliorate SCFA metabolic disorders induced by CIH. a Partial least squares discrimination analysis (PLS-DA). b Score plot of the OPLS-DA model. c – j Concentrations of SCFAs in each group. c Total SCFAs. d Acetic acid. e Butyric acid. f Isovaleric acid. g Valeric acid. h Caproic acid. i Propionic acid. j Isobutyric acid. * P < 0.05, ** P < 0.01, compared to the Control group; # P < 0.05, ## P < 0.01, compared to the CIH group

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Inhibition, Control

Journal: Applied Microbiology and Biotechnology

Article Title: Inhibiting the CB1 receptor in CIH-induced animal model alleviates colon injury

doi: 10.1007/s00253-024-13216-0

Figure Lengend Snippet: Inhibition of CB1 receptor promotes the production of intestinal tight junction proteins under CIH conditions and reduces intestinal inflammation and metabolic endotoxemia. a – c Western blot to detect the expression of ZO-1 and Occludin in colon. d , e RT-PCR to detect the mRNA expression of ZO-1 and Occludin in colon. f – h RT-PCR to detect IL-1β ( f ), TNF-α ( g ), and IL-10 mRNA ( h ). i Serum LPS level detected by ELISA (eu, ELISA units). * P < 0.05, ** P < 0.01, compared to the Control group; # P < 0.05, ## P < 0.01, compared to the CIH group

Article Snippet: CB1 receptor antagonist AM251 was purchased from Topscience (Shanghai, China, No: T1915), and rabbit anti-mouse Occludin (GB111402) and ZO-1 (GB11149) antibodies were purchased from Servicebio (Wuhan, China); rabbit anti-mouse CB1R antibody was purchased from Thermo Fisher (Waltham, MA, USA).

Techniques: Inhibition, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: bioRxiv

Article Title: The retinal light response is modulated by an mGluR5-mediated retrograde signal from ON-bipolar cells to photoreceptors

doi: 10.1101/2025.11.13.687902

Figure Lengend Snippet: The CB1 agonist ACEA reverses the effect of the mGluR5 antagonist MTEP at dim light intensities. A , Maximal scotopic a- and b-wave amplitudes of control- (black, n=9) and MTEP + ACEA (purple, n=14) -injected mice, plotted as a function of flash intensity in log (sc cd•s/m 2 ). For comparison, the a- and b-wave amplitudes for each drug alone, shown in and C are indicated in dotted lines (MTEP, green; ACEA, cyan). B , Normalized b-wave amplitudes of control (black) and MTEP + ACEA (purple), plotted as a function of flash intensity in log (sc cd•s/m 2 ). For comparison, the normalized b-wave amplitudes for each drug alone, shown in and D are indicated in dotted lines (MTEP, green; ACEA, cyan).

Article Snippet: Two rabbit monoclonal anti-CB1R antibodies were used, one from Cell Signaling Technologies (CST antibody; catalog # 93815) and one from Synaptic Systems (SySy antibody; Goettingen, Germany; catalog # 258 008) both of which have been validated on CB1R-knockout tissue ( ; ).

Techniques: Control, Injection, Comparison

Journal: bioRxiv

Article Title: The retinal light response is modulated by an mGluR5-mediated retrograde signal from ON-bipolar cells to photoreceptors

doi: 10.1101/2025.11.13.687902

Figure Lengend Snippet: CB1 receptors are expressed in the synaptic layers of the retina. A , Localization of CB1R in the mouse retina (CST antibody). B , Double labeling in the outer plexiform layer for CB1R (red, SySy antibody) and PNA (green), a marker of cone synaptic terminals. The scale bars correspond to 20 μm in A and 5 μm in B . Abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.

Article Snippet: Two rabbit monoclonal anti-CB1R antibodies were used, one from Cell Signaling Technologies (CST antibody; catalog # 93815) and one from Synaptic Systems (SySy antibody; Goettingen, Germany; catalog # 258 008) both of which have been validated on CB1R-knockout tissue ( ; ).

Techniques: Labeling, Marker